Peptide reconstitution calculator
Work out concentration, how much to draw and where that lands on a U-100 syringe, from your vial size and the volume of diluent you add.
Draw to
–units
Enter an amount to calculate
–Concentration
–Per syringe unit (0.01 mL)
–Measures per vial
How the math works
Reconstitution is simple division. Once the powder is dissolved, every 0.01 mL of liquid contains the same amount of peptide.
- Concentration = peptide in vial ÷ diluent volume
10 mg ÷ 2 mL = 5 mg/mL (5,000 mcg/mL) - Volume to draw = amount wanted ÷ concentration
250 mcg ÷ 5,000 mcg/mL = 0.05 mL - Syringe units = volume × 100 on a U-100 syringe
0.05 mL × 100 = 5 units
Adding more diluent makes the solution weaker, which means larger, easier-to-read volumes for small amounts. Less diluent makes it stronger and the volumes smaller.
Before you calculate
- The calculator does the arithmetic only. It does not recommend amounts for any compound.
- Check the exact peptide content on the vial label and batch certificate of analysis.
- U-100 syringes hold 100 units per mL, so 1 unit = 0.01 mL.
- Volumes under about 2 units are hard to measure accurately. Add more diluent to work with larger volumes.
- New to this? Follow our step-by-step reconstitution guide (with diagrams).
- Read our guides on reconstitution basics and storage and stability.
For laboratory research use only. This tool is not medical advice and is not a guide to human or animal dosing.